Article

The present study was designed to investigate the effect of extremely low
frequency (ELF) magnetic field (MF) on spinal cord injury (SCI)-induced
osteoporosis in rats. Adult male Wistar rats (n = 24) were equally divided into
sham, SCI, and SCI+MF groups. Complete transection of spinal cord (thoracic 11
vertebra) was surgically performed under anesthesia, whereas in the sham group
only laminectomy was done. Post-SCI day 1, rats were either exposed (2 h/d x 8
wk) to ELF-MF (17.96 micro-Tesla, 50 Hz; SCI+MF group) or sham exposed (SCI
group). Basso, Beattie, and Bresnahan (BBB) score was recorded weekly. All the
rats were sacrificed 8 wk post-SCI; tibia and femur bones were isolated for the
analysis of bone mineral content (BMC; total calcium [Ca], phosphorus [P], carbon
[C]), bone mineral density (BMD), and biochemical status (osteocalcin, collagen
I, alkaline phosphatase). The BBB score decreased post-SCI, which partially
recovered after ELF-MF. In SCI rats, there was a statistically significant
decrease in BMC, Ca, P, C, BMD, and biochemical level in both the bones as
compared with the sham group, which was attenuated in SCI+MF rats except the C
content. Electron microscopic study revealed the enhancement of microstructural
composition and compactness in cortical and trabecular parts of treated bones.
The results suggest that the chronic (2 h/d x 8 wk) ELF-MF exposure (17.96
micro-Tesla, 50 Hz) to SCI rats is effective in attenuating SCI-induced
osteoporosis.

Langue : ANGLAIS